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KMID : 0350519930460041607
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1607 ~ p.1617
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Determination of the Optimum Dosage of 8-Methoxypsoralen and Ultraviolet A Light for Inducing DNA Interstrand Cross- Links
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Abstract
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Photochemotherapy using the photosensitizer and ultraviolet A light is a relatively new and potentially selective experimental approach to the treatment of malignant neoplasms.
The combination of 8-methoxypsoralen and ultraviolet A light(320-400 nm) is used in the treatment of psoriasis, a hyperproliferative disease of the skin, by means of its antiproliferative and cytotoxic effects associated with photoaddition of
8-methoxypsoralen.
In this study, interstrand cross-liks of HindⅢ-linearized plasmid DNA fwere induced after in vitro treatment with 8-methoxypsoralen and ultraviolet A light. In addition, 8-methoxypsoralen was treated at various concentration from 0.0005 to
15.0㎍/μl
to determine the usable dosage of 8-methoxypsoralen and irradiation dosage of ultraviolet A light for its therapeutic use in gynecologic malignant diseases.
The double stranded DNA formed by 8-methoxypsoralen photoiduced cross-links were appeared from the 0.128㎍/μl of 8-methoxypsoralen using 20 KJ/㎡ of ultraviolet A light dose, showing increased proportion of double stranded DNA with itsincreasing
doses
and vice versa.
These results suggested that 0.128㎍/μl or more tissue concentrations of 8-methoxypsoralen and 20 KJ/㎡ target tissue dose of ultraviolet A light will be able to be recommended for the treatment of malignant neolpasms.
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